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The University of Tennessee

College of Arts & Sciences

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Barry D. Bruce, Ph.D.

See Also: Bruce Lab

Research Statement

My lab works concurrently on two aspects of membrane biochemistry related to photosynthesis: Chloroplast protein import and Applied photosynthesis.

  • Chloroplast protein import: I have a long-standing interest in on the biogenesis of organelles and in particular the family of organelles specific to plants, known as plastids. This family includes a divers set of organelles that perform an extensive set of biochemical reactions including the process of photosynthesis, which takes place in chloroplasts. This diverse metabolic activity is the enabled via the import of several thousand nuclear encoded proteins from the cytosol. This targeting and import process is critical to many if not most of the key metabolic processes associated with plants. WE are specifically interested in how chloroplast destined precursors are targeted, recognized and translocated into the plastid via two translocons, TIC and TOC, that are found in the chloroplast inner and outer envelope membranes, respectively. This process is mediated by selective recognition of the transit peptide by one or more of the TOC components. We are working to explain the biochemistry and biophysics of how these proteins are translocated across the plastid membranes.

  • Applied photosynthesis: A second area of research is how we may exploit the ultra efficient process of solar energy capture and conversion of natural photosynthesis into hybrid devices that will provide either direct photovoltaic electricity or be stored in some form of fuel such as molecular hydrogen. To date we have help design build and characterize novel: 1) luminescent solar concentrators, 2) hybrid Bio-organic thin-film photovoltaic devices, 3) hydride bio-inorganic photovoltaic devices, and 4) bio-Pt hydrogen evolving nanoparticles.



Selected Publications

Research Papers

Myers, N. and Vaughn, M., Mahbubani, P., Paquet, C., and Bruce, B. D. (2009) Optimization of expression of a thermophilic cyanobacterial cytochrome c6 in E. coli and engineering of additional thermal enhancements. to be submitted

Reddick, L. E., Chotewutmontri, P., Campbell, I., McWilliams, D. R., and Bruce, B.D. (2009) In Vitro and In Organeller Transit Peptide Recognition Reveals Sequence Specificity. to be submitted

Reddick, L. E., Campbell, I., Wright, S. J.,  and Bruce, B.D. (2009) The Toc Clock: Oligomerization of the Toc GTPases is modulated by Nucleotide and Transit Peptide. to be submitted

Mukherjee, D., Vaughn, M., Khomami, B., and Bruce, B.D. (2009) Controlling the morphological assembly of Photosystem I deposited on thiol activated Au substrates. Biophysics J. in preparation

Iwuchukwu, I., Vaughn, M., Myers, N., O’Neill, H., Frymier, P., & Bruce, B. D. (2010) Self-assembled Photosynthetic Nanoparticle for Cell-free Hydrogen Production. Nature Nanotechnology, 5, 73-79.

Mershin, A., Matsumoto, K., Kaiser, L., Yu, D., Vaughn, M., Nazeeruddin, M., Bruce, B.D., Graetzel, M., and Zhang, S. (2010) Self-Assembled Photosystem-I Photovoltaics On Nanostructured TiO2 And ZnO. Nature Nanotechnology in review

Mulder, C.L., Theogarajan, L., Currie, M., Mapel, J.K., Vaughn M., Willard, P., Moss, M.W., C.E. McLain, C.E, Morseman, J.P., Bruce, B.D., M.A. Baldo (2009) Luminescent Solar Concentrators Employing Phycobilisomes. Advanced Materials 21:1-5

Matsumoto, K., Vaughn, V., Bruce, B.D., Koutsopoulos, S & Zhang, S. (2009) Designer Peptide Surfactants Stabilize Functional Photosystem-I Membrane Complex In Aqueous Solution For Extended Time. J. Phys. Chem B. 113:1, 75-83.

Reddick, L, Chotewutmontri, P., Crenshaw, W., Dave, A., Vaughn, M., & Bruce, B.D. (2008) Nano-scale Characterization of the Dynamics of the Chloroplast Toc Translocon. Methods in Cell Biology, 95, 365-387.

Gaysinsky, S., Taylor T.M., Davidson, P.M., Bruce, B.D., and Weiss, J. (2008) Antimicrobial efficacy of eugenol microemulsions in milk against Listeria monocytogenes and Escherichia coli O157:H7 J. Food Prot. 71:6: 1256-1261.

Reddick, E. L., Vaughn, M., Wright, S. J., Campbell, I., and Bruce, B.D. (2007) in vitro comparative kinetic analysis of the chloroplast Toc GTPases. J. Biol. Chem. 282(15):11410-11426.

Gülseren, I., Güzey, D., Bruce, B.D. and Weiss, J. (2007) Structural and Functional Changes in High-intensity Ultrasonicated Bovine Serum Albumin. Ultrasonics Sonochemistry, 14: 173-183.

Taylor T.M., Gaysinsky, S., Davidson, P.M., Bruce, B.D., and Weiss, J. (2007) Characterization of Antimicrobial-Bearing Liposomes by Zeta-Potential, Vesicle Size, and Encapsulation Efficiency. Food Biophysics  2:1, 1-9. 

Papalia, G., Leavitt, S., Bynum, M., Katsamba, P., Wilton, R., Qiu, H., Steukers, M., Wang. S., Bindu, L., Phogat, S., Gianetti, A., Ryan, T., Victoria, A., Pudlak, V., Matusiewicz, K., Michelson, K., Nowakowski, A., Pham-Baginski, A., Brooks, J., Tieman, B., Bruce, B.D., Vaughn, M., Baksh, M., Cho, Y., Lindquist,K., De Wit, M., Smets, A., Vandersmissen, J., Michiels, L., , and Myszka, D. (2006) Comparative Analysis Of Ten Small Molecules Binding To Carbonic Anhydrase II By Different Investigators Using Biacore Technology. Anal. Biochem. 359(1):94-105.

Yu, A.A., Stoney, P.R., Norvillez, J.E., Vaughn, M., Pacsial, E.J., Bruce, B.D., Baldo, M., and Stellacci, F. (2006) A Simple Atomic Force Microscopy Method The Visualization Of Polar And Non-Polar Parts Organic Films. J. Exp. Nanoscience, 1, 63-73.

Güzey, D., Gülseren, I., Bruce, B.D. and Weiss, J. (2006) Interfacial Properties and Structural Conformation of Thermosonicated Bovice Serum Albumin. Food Hydrocolloids 20:5, 669-677.

Gaysinsky, S., Davidson, P. M., Bruce, B.D., and Weiss, J. (2005) Growth inhibition of Escherichia coli O157:H7 and Listeria monocytogenes by Carvacrol and Eugenol Encapsulated in Surfactant Micelles. Stability and Antimicrobial Efficiency of Eugenol Encapsulated in Surfactant Micelles. J. Ag. Food Chem., 68, 2556-2566.

Taylor, M. T., Davidson, P. M., Bruce, B.D., and Weiss, J. (2005) Ultrasonic Spectroscopy and Differential Scanning Calorimetry of Liposomal Encapsulated Nisin. J. Ag. Food Chem. 53, 8722-8728.

Bhushan S.,  Stahl, A, Nilsson, S., Lefebvre, B., McWilliams, D., Wright S.J., Seki, M., Liberles, D.A., Shinozaki, K., Bruce B.D., Boutry, M., and Glaser E (2005) Molecular Characterization of a isoenzyme of an of the targeting peptide degrading protease, PreP2- catalysis, subcellular localization, expression and evolution. Plant & Cell Physiology, 46(6):985-996.

Kiley, P., Zhao, X., Vaughn, M., Baldo, M.A., Bruce, B.D., and Shuguang Zhang (2005) Self-assembling Peptide Detergents Stabilize Isolated Photosystem I on a Dry Surface for an Extended Time.  PloS Biology, 3(7), e230-237.

Gaysinsky, S., Davidson, P.M., Bruce, B.D., and Weiss, J. (2005) Stability and Antimicrobial Efficiency of Eugenol Encapsulated in Surfactant Micelles as Affected by Temperature and pH, J. Food Prot.68(7): 1359-1366.

Strader, M.B., VerBerkmoes, N.C., Tabb, D.L., Connelly, H.M., Barton, J.W., Bruce, B.D., Pelletier, D.A., Davison, B.H., Hettich, R.L., Larimer, F.W., Hurst, G. (2004) Characterization of the 70S ribosome from Rhodopseudomonas palustris using an integrated "top-down" and "bottom-up" mass spectrometric approach. J.  Proteome Res. 3(5): 965-978.

Evans, B.R., O'Neill, H.M., Hutchens, S.A., Bruce, B.D., and Greenbaum E  (2004) Enhanced photocatalytic hydrogen evolution by covalent attachment of plastocyanin to photosystem I. Nano Lett. 4(10): 1815-1819.

Das, R., Kiley, P.J., Segal, M., Norville, J., Yu, A.A., Wang, L.Y., Trammell, S.A., Reddick, L.E., Kumar, R., Stellacci, F., Lebedev, N., Schnur, J., Bruce, B.D., Zhang, S.G., and Baldo, M. (2004) Integration of photosynthetic protein molecular complexes in solid-state electronic devices. Nano Lett. 4(6): 1079-1083.

Were LM, Bruce B.D, Davidson P.M., and Weiss J. (2004) Encapsulation of nisin and lysozyme in liposomes enhances efficacy against Listeria monocytogenes J. Food Prot. 67(5): 922-927.

Boland JS, Davidson P.M., Bruce B.D., and Weiss J. (2004) Cations reduce antimicrobial efficacy of lysozyme-chelator combinations.  J. Food Prot. 67(2): 285-294 FEB 2004

Were L.M., Bruce, B.D., Davidson, P.M., Weiss J. (2003) Size, stability, and entrapment efficiency of phospholipid nanocapsules containing polypeptide antimicrobials. J. Agric. Food Chem. 51(27): 8073-8079.

Moberg P., Stahl, A., Bhushan, S., Wright, S.J., Eriksson A., Bruce, B.D., and Glaser, E. (2003) Characterization of a novel zinc metalloprotease involved in degrading targeting peptides in mitochondria and chloroplasts Plant J. 36(5): 616-628.

Bhushan, S., Lefebvre, B., Stahl, A., Wright, S.J., Bruce B.D., Boutry, M, and Glaser, E. (2003) Dual targeting and function of a protease in mitochondria and chloroplasts EMBO Rep.  4(11): 1073-1078.

Kumaraguru, U., Gouffon, C., Ivey, R., Rouse, B.T. and Bruce, B.D. (2003) Antigenic peptides complexed to phylogenically diverse hsp70s induce differential immune responses. Cell Stress & Chaperones, 8(2): 134-143.

VerBerkmoes, N.C., Hettich, R.L., Bruce, B.D., Nguyen, R., and Savage, T.L. (2002) One- and two-dimensional LC/MS/MS analysis of Arabidopsis thaliana proteome. LC/GC (North America) 10-11.

Schleiff, E., Sveshnikova,N., Tien, R., Soll, J., Wright, S., Dabney-Smith, C., Subramanian, C., and Bruce, B.D. (2002) Structural and nucleotide requirements for transit peptide recognition by the cytosolic domain of the receptor, Toc34 and the chloroplast translocation machinery. Biochemistry 41: 1934-1946.

Kumaraguru, U., Bruce, B.D., and Rouse, B.T. (2002) Immunization with a chaperone-peptide complex induces low avidity CTLs providing transient protection against HSV infection. J. Virology76: 136-141.

Lee, S.-Y., Dabney-Smith, C., Hacker, D.L., and Bruce, B.D. (2001) Membrane Activity of the SCPMV Coat Protein: The Importance of Basic Amino Acids, Helix-forming Potential, and Lipid Composition.. Virology 291(2): 299-310.

Peng, Z., Staub, J.M., Serino, G., Kwok, S.F., Kurepa, J., Bruce, B.D., Vierstra, R.D., Wei., N., Deng, X-W., (2001) The cellular level of PR500, a protein complex related to the 19S regulatory particle of the proteasome, is regulated in response to stresses in plants. Mol. Biol. Cell 12(2): 383-392.

Millsaps, J.I., Bruce, B.D., Lee, J.W., and Greenbaum, E., (2001) Nanoscale photosynthesis: Photocatalytic production of hydrogen by platinized photosystem I reaction centers. Photochem. Photobio. 73: 630-636.

Subramanian, C., Ivey, R.A., and Bruce, B.D. (2001) Cytometric analysis of an epitope-tagged transit peptide bound to the chloroplast translocation apparatus Plant J. 23: 349-363.

Kumaraguru, U., Nair, S., Rouse, R.J., Bruce, B.D., and Rouse, B.T. (2000) Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization. J. Immun. 165: 750-759.

Ivey, R.A., Subramanian, C., and Bruce B.D. (2000) Identification of a Hsp70 recognition domain within the Rubisco Small Subunit Transit Peptide Plant Physiol. 122: 1289-1299.

Ivey, R.A. and Bruce B.D. (2000) In vivo and in vitro interaction between a chloroplast transit peptide and dnaK. Cell Stress & Chaperones 5: 62-71.

Dabney-Smith, C., van den Wijngaard, P., Treece, Y., Vrendenberg, W., and Bruce, B.D. (1999) The C-terminus of a chloroplast precursor modulates interaction with the translocation apparatus and PIRAC. J. Biol. Chem. 274: 32351-32359.

van den Wijngaard, P., Dabney-Smith, C., Bruce, B.D., and Vrendenberg, W., (1999) The mechanism of inactivation of a 50 pS envelope anion channel during chloroplast protein import.  Biophys. J. 77: 3156-3162.

Miltenberger, R.J., Mynatt, R.L. Bruce, B.D., Wilkinson, W.O., Woychik, R.P. and Michaud, E. J (1999) An agouti mutation lacking the basic domain induces yellow pigmentation but not obesity in transgenic mice. Proc. Natl. Acad. Sci. USA 96: 8579-8584.

Kausch, A., Owen, T.P., Narayanswami, S., and Bruce, B.D. (1999) Organelle isolation by magnetic immuno-absorption. Biotechniques 26: 336-343.

Bruce, B.D. (1998) The Role of Lipids in Plastid Protein Transport.  Plant Mol. Biol. 38: 223-246.

Bruce, B.D., and Churchich, J. (1997) Characterization of the Molecular Chaperone Function of the Heat Shock Cognate 70-Interacting Protein, Hip. Eur. J. Biochem. 245: 738-744.

Pinnaduwage, P.D. and Bruce B.D. (1996) In vitro interaction between a chloroplast transit peptide and chloroplast outer membrane lipids is sequence specific and lipid-class dependent. J.  Biol. Chem. 271: 32907-32915.

Keegstra, K., Bruce, B.D., Li, H.-M., and Perry, S. (1995) Targeting of proteins into chloroplasts. Physiol. Plant. 93: 157-162.

Kausch, A. and Bruce, B.D. (1994) Isolation and immobilization of various plastid forms by magnetic immunoabsorbtion. Plant J. 6: 767-779.

Markwell, J., Bruce, B.D., and Keegstra, K. (1992) Isolation of a Carotenoid-Containing Sub-membrane Particle from the Chloroplast Outer Envelope of Pea (Pisum sativum). J. Biol. Chem. 267: 13933-13937.

von Heijne, G., Hirai, T., Klösgen, R.B., Steppuhn, J., Bruce, B.D., Keegstra, K., and Herrmann, R. (1991) CHLPEP- A Database of Chloroplast Transit Peptides. Plant Mol. Biol. Rep. 9: 104-126.











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