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Lab meeting

Becker Lab Meeting Schedule for Summer, 2005

Microbiology Conference Room

Mondays at 12:00

Bring your lunch along with your data!

* Since everyone should bring their own lunch, snacks should be something simple to share for dessert.

Becker Lab Personnel (Updated  5/18/2005)

Lab rules

General Rules and Protocols
1. General lab rules
a. Follow safety rules (see attachment in the lab)
b. Return reagents, glassware, etc. to proper place after use.
c. Help maintain the catalogs of chemical, lab strains, etc.
d. Report broken equipment to person in charge.
e. Place all dirty glassware in proper vats (filled with soapy water)
f. Date packing slips. Inform person who placed order.
g. Take accurate phone messages. Deliver them to appropriate person promptly.
h. Consider your Becker lab-mates as a team (See attached flyer on team- building). Be a “team player.” Be considerate!
i. Carry out your assigned lab duties (See the list in the lab) enthusiastically and in a timely manner.
j. Adhere to the rules for radioactive material usage, clean work areas and clean instruments, and so forth(see attached memo on radioactive hood).
k. Be extremely cautious with valuable material, especially our synthetic peptides which are often the only source on earth. If in doubt, ask Dr. Becker.
l. Prepare Monthly Report and submit on the first day of the month.

2. Lab Philosophy
(See attached letter from JMB to Becker Lab for overall work ethic philosophy)
(See attached article on Joy of Science. Incorporate these insights into your performance.)
a. Technician- 40 h.
b. Graduate student- 70-80 h.
c. Post-doc- ??

3. Lab Notebooks
a. Write up experiments according to “Rules for lab notebooks” (see attachment in the lab).
b. Write up introduction, purpose, protocols of experiments before doing them.
c. Record all observations made and alterations to protocols that occur.
d. Don’t vary more that one condition simultaneously when repeating experiment.
e. Maker all experiments count: visualize their incorporation as figure of table in published document.

4. Lab meetings (See attachment on The Research Team Meeting.)
a. Forum of ideas.
b. Present research, both experiments that work and those that don’t!
c. Present journal articles, ideas, and techniques gleaned from literature.
d. Review housekeeping, air problems, voice concerns.
e. Participate actively.
f. Read the listed articles on yeast cell biology (See attached list in the lab). Know this material.

5. Restriction 
a. No latex 
b. No foods containing peanut, nuts, egg, soy, cockroach, ants, elephant, etc.
                            or Amy will butcher you!!!

Lab jobs

Here is the current list of assigned lab jobs. Please make sure you are a good lab citizen and actually DO YOUR JOB. If there are problems with equipment (i.e. broken microfuge, burned out microscope bulbs, etc.) or housekeeping (i.e. hoods, weighing area), please contact the appropriate person listed below. If you have a question about your job responsibilities, please ask Melinda.

May 23, 2005

Houjian Cai

1. Restriction enzyme inventory

2. Recycle paper in E308 main lab and computer room

3. Clean dishpan in the sink in E308 (i.e. dump it and replace the water and bleach before it starts to smell)

4. Maintain ampicillin stocks

5. Maintain yeast deletion series

Melinda Hauser

1. Annual equipment inventory

2. Maintain service contracts

3. Coordinate and track purchases both in the lab and with BBO

4. Scintillation counters – calibration and maintenance.

5. Milli-Q Water system.  Check operation and replace cartridge when necessary.  Be sure the system is shut-down before any planned water outage.

6. Equipment repairs through BSF

Agnieszka Janiak

1. Fluorescence microscope maintenance and repair

2. File and maintain collection of lab journals

3. Check and clean waterbaths (make sure temperature is correct and that they are not running dry) and dry baths (make sure temperature is correct).

4. Remind Steve and George  that it is Radiation/Hazardous Waste Wednesday

5. Maintain common stocks of agar butts

Sarah Kauffman

1. Centrifuges and microfuges, including rotor maintanence

2. –20 Freezers.  Organize and coordinate defrosting when necessary.

3. Clean dishpan in the sink in E314 (i.e. dump it and replace the water and bleach before it starts to smell)

4. PAGE supplies and equipment (including buffers, markers, plates, etc.)

5. Monthly maintenance of -80C freezer air filters

Heejung Kim

1. Maintain balance area in E314

2. Maintain pH meter

3. Protease inhibitor stocks

4. Autoclave 250 ml flasks for general lab use – coordinate with Amy

5. Cold room organization.  Make sure that common areas are clean and uncluttered and that everything is properly labeled

Byung-Kwon Lee

1. Thermocyler and supplies.  Make sure we have tubes, polymerase, etc.

2. Agarose electrophoresis area.  Make sure we have 50X TAE and that everything is organized and in working order.

3. Recycle paper in E314

4. Laminar flow hood (E314).  Keep it clean and organized.

Yong-Hun Lee

1. Microscope maintenance

2. FAB-MS of peptide

3. Peptide catalog.  Log-in and store peptides received from Naider Lab and other collaborators

4. Aliquot and freeze competent cells

Tom Masi

1. General-purpose lab supply inventory (i.e. petri dishes, tubes, etc.) Order items as needed

2. Unpack shipments, log-in arrival and give packing slips to BBO

3. Media and chemical reagent inventory

4. Order supplies listed on “Supply Board”

5. Maintain -80 freezer inventory and strain databases

6. Catalog maintenance.  Alphabetize catalogs.  Discard outdated or duplicate material

Lindsey Mills

1. Copying and errands for JMB and Sandy

2. Update chemical inventory (with Tom)

3. Media preparation as needed

Steve Minkin

1. Radiation Safety Officer.  Complete monthly radiation reports, collect and distribute dosimeters, inventory radioactive materials.  Verify that we are in compliance with policy

2. Radiation/Chemical disposal.  Make sure that waste is properly labeled and removed on Wednesdays.

3. Monthly radiation swabs in C303.

4. Make sure radioactive work area and radioactive sink in C303 are clean and organized.  Dispose of “orphan” scintillation vials abandoned on counters

5. Lab coat transport to and from Neyland Annex

Josh Ogle

1. Copying and errands for JMB and Sandy

2. Update chemical inventory (with Tom)

3. Media preparation as needed

Cagdas Son

1. Computer software, maintanance and other problems

2. Chemi Doc imaging system (maintenance, software, etc.)

3. Digital camera.  Make sure that you know where it is and that it has functional batteries

4. HPLC maintenance and repair.  Coordinate with service tech under terms of the service contract

5. Radioactive hood (E313 and C303).  Coordinate waste disposal with Steve.

6. Vacuum pumps, including the Savant speed-vac.  Verify that pumps are in working order, change oil as needed.

George Umanah

1. Chemical fume hood (E308).  Verify that everything is properly labeled and that we are in compliance with health and safety regulations

2. UV/VIS Spectrophotometer

3. Skatron cell harvester maintenance – includes inventory of supplies (filter mats, scintillation vials and cocktail)

4. Hazardous waste.  Disposal on Wednesdays (coordinate with Minkin), reports and inventory

5. Incubators.  Make sure they are clean and in working order (temperature, shaker, water tray, etc.)  Dispose of orphan plates or cultures

Amy Wiles

1. Back-up Radiation Safety Officer while Steve is out of the lab.

2. Maintain balance area in E308

3. Maintain phosphorimager screen

4. Maintain sheared salmon sperm DNA stocks

5. Autoclave 250 ml flasks for general lab use – coordinate with Heejung

Protocols

Yeast transformation