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The University of Tennessee

University of Tennessee Department of Classics

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FAQ - Frequent Asked Questions

How much do we charge?

  • $4.25 per sequence for UT, $3.00 per genescan for UT.
  • 3.00 load-only sequencing for UT.

 How much DNA do I need to sequence?

  • Plasmids need 450ng/rxn minimum in <15uL’s H2O
  • PCR fragments need 10ngs per 100 base pairs in 15uL’s or less H2O

When can I get my results?

  • If here in the lab by 11:00 am, then most likely the next morning.

What fragment analysis dyes should I order?

  • Dye set D (3unknowns per well) = 6FAM, HEX, NED, ROX (size std.)
  • Dye set G5 (4unknowns per well) = 6FAM, VIC, NED, PET, LIZ (size std.)