BCMB 515
VECTOR SEQUENCES
In this section, we have established links to
information about the two vectors that we will be using in the course. For
pUC18, we have links to plasmid DNA maps, lists of restriction enzyme sites and the full DNA
sequence. Click on the appropriate title to get the information you
want!
A. pUC18
B. pCR2.1 (Invitrogen)
C. Data for 515 Groups
| Group 1 | Group 3 | Group 5 |
| Group 2 | Group 4 | Group 6 |
pUC18 DNA: Location of Restriction Endonuclease Sites
In this section are listed all enzymes that cut pUC18 one or two times. Also listed are enzymes which do not cut the vector. Other restriction sites should be assumed to occur greater than two times within the plasmid.
| AatII | 1 | 2617 | |
| Acc65I/KpnI | 1 | 435 | |
| AccI | 1 | 414 | |
| AhdU/Eam1105I | 1 | 1694 | |
| AflIII | 1 | 806 | |
| AlwNI | 1 | 1217 | |
| ApoI | 1 | 396 | |
| AvaI | 1 | 431 | |
| BamHI | 1 | 426 | |
| BanII | 1 | 402 | |
| BcgI | 1 | 2215 | |
| BpmI | 1 | 1784 | |
| BsaI | 1 | 1766 | |
| BspMI | 1 | 410 | |
| BsfI | 1 | 1779 | |
| Ecl136II/SacI | 1 | 441 | |
| EcoO109I | 1 | 2674 | |
| EcoRI | 1 | 447 | |
| HincII | 1 | 414 | |
| HindIII | 1 | 396 | |
| KasI/NarI | 1 | 235 | |
| Nde | 1 | 183 | |
| PstI | 1 | 407 | |
| SalI | 1 | 414 | |
| SapI | 1 | 683 | |
| ScaI | 1 | 2177 | |
| SmaI/XmaI | 1 | 431 | |
| SphI | 1 | 402 | |
| Sse8387I | 1 | 409 | |
| SspI | 1 | 2501 | |
| SbaI | 1 | 420 | |
| XmnI | 1 | 2294 | |
| BglI | 2 | 245 | 1813 |
| BsmBI | 2 | 51 | 2683 |
| BsrDI | 2 | 1753 | 1935 |
| DrdI | 2 | 908 | 908 |
| Eco57I | 2 | 1333 | 2381 |
| FspI | 2 | 256 | 1919 |
| Psp1406I | 2 | 1924 | 2297 |
| PvuI | 2 | 276 | 2066 |
| PvuII | 2 | 306 | 628 |
| TfiI | 2 | 641 | 781 |
The following enzymes do not cut pUC18 DNA:
AflII, AgeI, ApaI/Bsp120I, AscI, AvrII, BbsI, BclI,
BglII, BlpI, BsaAI,
BsaBI, BsseRI, BsgI, BsiWI, BsmFI, BsmI, BspDI/ClaI,
BspEI, BstGI, BssHII,
Bst1107I, BstBI, BstEII, BstXI, Bsu36I, DraIII, DsaI,
EagI, Eco47III, EcoNI, EcoRV, FseI, HpaI, MfeI, MluI, MscI, NaeI/NgoMI, NcoI,
NheI, NotI, NruI, NsiI/Pps10I, PacI, PaeR7I/XhoI, PflMI, PmeI, PmlI, PpuMI,
PshAI, RsrII, SacII, SexAI, SfiL, SgfI, SgrAI, SnaBI, SpeI, SrfI, StuI, StyI,
SwaI, Tth111I, XcmI
DEFINITION pUC18 cloning
vector.
ACCESSION L08752
KEYWORDS .
SOURCE Synthetic construct
DNA.
ORGANISM Synthetic construct
Artificial sequences.
REFERENCE 1
(sites)
AUTHORS Norrander,J.M., Kempe,T. and Messing,J.
JOURNAL Gene 26,
101-106 (1983)
MEDLINE 84159483
REFERENCE 2 (bases 1 to 2686)
AUTHORS
Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
(1991)
COMMENT These data and their annotation were supplied to GenBank by
Will
Gilbert under the auspices of the GenBank Currator Program. pUC18
-
Cloning vector
ENTRY PUC18 #TYPE DNA CIRCULAR
TITLE pUC18 - Cloning
vector
DATE 17-SEP-1986
#sequence 16-DEC-1986
ACCESSION
VB0025
SOURCE artificial
COLLECTION ATCC 37253
REFERENCE
#number
1
#authors Norrander J., Kempe T., Messing J.
#journal Gene (1983) 26:
101-106
REFERENCE
#number 2
#authors Pouwels P.H., Enger-Valk B.E.,
Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment
vector I-A-iv-20
COMMENT
Assembled from pUC19 and M13mp18 by F. Pfeiffer,
MPI,
Martinsried
Revised 16-DEC-1986 by F. Pfeiffer:
2308/9 'AT' to
'TA' to match revised sequence of PBR322
The strand shown contains the
beta-galactosidase mRNA sequence
including the multiple cloning site of
M13mp18.
KEYWORDS
CROSSREFERENCE
#complement
VecBase(3):pUC18c
#parent
VecBase(3):pUC12,
VecBase(3):M13mp18,
VecSource(3):bGal18
#brother
VecBase(3):pUC19
#offspring
VecBase(3):pGEM3,
VecBase(3):pTZ18R,
VecBase(3):pIBI24, VecBase(3):pIBI30,
VecBase(3):pSP18,
VecBase(3):pT7T318,
VecBase(3):pT7T3A18
PARENT
Features of pUC18 (2686 bp)
residue
source
1- 447 6002-6448 M13mp18
1- 229 1069-1297 Lac-Operon
230- 286 1-
57 polylinker of M13mp18
289- 447 1303-1461 Lac-Operon
448- 547 2351-2252
(c) pBR322
548- 684 2210-2074 (c) pBR322
685-2686 4355-2354 (c)
pBR322
Conflict (cfl) and Mutations (mut):
pUC18 source
mut 1128 T C
3912 (c) pBR322
mut 1429 A G 3611 (c) pBR322
FEATURE
952-1740 1-789
Ap-R; b-lactamase
POLYLINKER
EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-PstI-SphI-HindIII
SELECTION
#resistance
Ap
#indicator beta-galactosidase
SUMMARY pUC18 #length 2686 #checksum
5464.
NCBI gi: 209211
FEATURES
Location/Qualifiers
source 1. .2686
/organism="Synthetic
construct"
/sequenced_mol="DNA"
BASE COUNT 659 a 684 c 677 g 666
t
ORIGIN
Synpuc18v Length: 2686 April 20, 1995
11:40 Type: N Check: 5464 ..
1 GCGCCCAATA
CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT
51
GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
101 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG
CTTTACACTT
151 TATGCTTCCG GCTCGTATGT TGTGTGGAAT
TGTGAGCGGA TAACAATTTC
201 ACACAGGAAA CAGCTATGAC
CATGATTACG AATTCGAGCT CGGTACCCGG
251 GGATCCTCTA
GAGTCGACCT GCAGGCATGC AAGCTTGGCA CTGGCCGTCG
301
TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC
351 CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG
AAGAGGCCCG
401 CACCGATCGC CCTTCCCAAC AGTTGCGCAG
CCTGAATGGC GAATGGCGCC
451 TGATGCGGTA TTTTCTCCTT
ACGCATCTGT GCGGTATTTC ACACCGCATA
501 TGGTGCACTC
TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC
551
CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC
601 CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG
CTGCATGTGT
651 CAGAGGTTTT CACCGTCATC ACCGAAACGC
GCGAGACGAA AGGGCCTCGT
701 GATACGCCTA TTTTTATAGG
TTAATGTCAT GATAATAATG GTTTCTTAGA
751 CGTCAGGTGG
CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA
801
TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
851 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT
ATTCAACATT
901 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG
CATTTTGCCT TCCTGTTTTT
951 GCTCACCCAG AAACGCTGGT
GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
1001 TGCACGAGTG
GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG
1051
AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT
1101 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC
AAGAGCAACT
1151 CGGTCGCCGC ATACACTATT CTCAGAATGA
CTTGGTTGAG TACTCACCAG
1201 TCACAGAAAA GCATCTTACG
GATGGCATGA CAGTAAGAGA ATTATGCAGT
1251 GCTGCCATAA
CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
1301
GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC
1351 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
AGCCATACCA
1401 AACGACGAGC GTGACACCAC GATGCCTGTA
GCAATGGCAA CAACGTTGCG
1451 CAAACTATTA ACTGGCGAAC
TACTTACTCT AGCTTCCCGG CAACAATTAA
1501 TAGACTGGAT
GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC
1551
CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
1601 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG
CCCTCCCGTA
1651 TCGTAGTTAT CTACACGACG GGGAGTCAGG
CAACTATGGA TGAACGAAAT
1701 AGACAGATCG CTGAGATAGG
TGCCTCACTG ATTAAGCATT GGTAACTGTC
1751 AGACCAAGTT
TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
1801
AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA
1851 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC
CCGTAGAAAA
1901 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
TCTGCGCGTA ATCTGCTGCT
1951 TGCAAACAAA AAAACCACCG
CTACCAGCGG TGGTTTGTTT GCCGGATCAA
2001 GAGCTACCAA
CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
2051
ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
2101 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT
GTTACCAGTG
2151 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
ACCGGGTTGG ACTCAAGACG
2201 ATAGTTACCG GATAAGGCGC
AGCGGTCGGG CTGAACGGGG GGTTCGTGCA
2251 CACAGCCCAG
CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
2301
CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
2351 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG
AGGGAGCTTC
2401 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC
CTGTCGGGTT TCGCCACCTC
2451 TGACTTGAGC GTCGATTTTT
GTGATGCTCG TCAGGGGGGC GGAGCCTATG
2501 GAAAAACGCC
AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC
2551
CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
2601 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA
GCCGAACGAC
2651 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC
GGAAGA
AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTT
TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAG
GCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAA
ACAGCTATGACCATGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGC
TGGAATTCGGCTTAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAG
GGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGA
AAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAA
GAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCG
GCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCC
CGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGG
GGGCTCCCTTTAGGGTTCCGATTTAGAGCTTTACGGCACCTCGACCGCAAAAAACTTGATTTGGGTGATG
GTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAA
TAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCGCGGTCTATTCTTTTGATTTATAAGGG
ATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAATTCAGGGCGCAAGGGCTGCTA
AAGGAACCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGG
GCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGAT
AGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGT
TGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGA
TCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGG
CCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGT
GTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAA
CTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACG
TTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCG
CCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCT
ACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTG
TCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGC
GCGCATGCCCGACGGCGAGGATCTCGTCGTGATCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAA
AATGGCCGCTTTTCTGGATTCAACGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGT
TGGATACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTAT
CGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTGAAAAAGGA
AGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTT
TGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCA
CTTTTAAAGTTCTGCTATGTCATACACTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCG
GGCGCGGTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAA
CGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG
TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGAGTGACACCACGATGCCTGTAGCAATGCCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGA
TGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAA
ATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAG
GTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAA
ACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAA
CGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTT
TTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCA
AGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA
GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC
TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACC
GGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTAC
ACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACA
GGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA
TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG
CGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATAC
CGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG
General Description
DNA pCR2.1
Entire molecule length: 3906 bp
|
Enzyme |
# of cuts |
Positions |
|
Acc65I |
1 |
241 |
|
AciI |
49 |
19 29 85(c) 188(c) 270 326(c) 330 497 559(c) 573(c) 576(c) 604 631 746 885(c) 1010 1133(c) 1229 1332 1395 1489(c) 1553(c) 1654(c) 1657(c) 1897 1937(c) 1942 1992(c) 2008 2034 2149(c) 2258(c) 2385(c) 2500(c) 2546 2737(c) 2828(c) 3190 3199(c) 3334 3444(c) 3565(c) 3584(c) 3711(c) 3739(c) 3830 3851 3858 3901(c) |
|
AcsI |
3 |
284 300 958 |
|
AcyI |
3 |
1175 1423 2354 |
|
AflII |
1 |
292 |
|
AflIII |
2 |
993 3782 |
|
AluI |
22 |
56 120 215 237 249 475 690 729 947 1044 1097 1123 1169 1529 1987 2541 2604 2704 3225 3482 3618 3844 |
|
Alw44I |
2 |
2222 3468 |
|
AlwI |
17 |
248(c) 261 1244 1261 1291 1602 1667(c) 1846(c) 1848 2255 2259(c) 2576 3039(c) 3040 3136(c) 3138 3224 |
|
AlwNI |
2 |
1047 3373 |
|
AosI |
3 |
525 1525 2671 |
|
ApaI |
1 |
355 |
|
ApaLI |
2 |
2222 3468 |
|
ApoI |
3 |
284 300 958 |
|
AseI |
3 |
48 107 2719 |
|
AsnI |
3 |
48 107 2719 |
|
Asp700 |
1 |
2294 |
|
Asp718 |
1 |
241 |
|
AspEI |
1 |
2894 |
|
AspHI |
6 |
251 1536 1726 2226 2311 3472 |
|
AspI |
1 |
1541 |
|
AvaI |
1 |
333 |
|
AvaII |
3 |
1939 2530 2752 |
|
AviII |
3 |
525 1525 2671 |
|
BamHI |
1 |
253 |
|
BanI |
7 |
132 241 736 1174 1422 1457 2941 |
|
BanII |
4 |
251 355 706 1788 |
|
BbvI |
19 |
65 465 538 607 962(c) 1370(c) 1496 1538 1554(c) 1647(c) 2059 2460(c) 2851 3154(c) 3360(c) 3363(c) 3453 3872 3890 |
|
BcgI |
1 |
2356(c) |
|
BclI |
1 |
1264 |
|
BfaI |
7 |
260 346 624 1124 2701 3036 3289 |
|
BfrI |
1 |
292 |
|
BglI |
2 |
535 2776 |
|
BglII |
1 |
1259 |
|
BmyI |
11 |
251 355 706 1369 1462 1536 1726 1788 2226 2311 3472 |
|
BpmI |
1 |
2825 |
|
BsaAI |
3 |
777 996 1727 |
|
BsaBI |
3 |
314 1258 1282 |
|
BsaHI |
3 |
1175 1423 2354 |
|
BsaI |
1 |
2828 |
|
BsaJI |
7 |
137 425 1028 1232 1586 1855 3622 |
|
BsaWI |
6 |
987 1156 1454 2598 3429 3576 |
|
BsiEI |
9 |
270 330 506 746 1332 2376 2525 3448 3872 |
|
BsiHKAI |
6 |
251 1536 1726 2226 2311 3472 |
|
BsiYI |
9 |
35 558 884 1404 1948 3304 3583 3749 3767 |
|
BslI |
9 |
35 558 884 1404 1948 3304 3583 3749 3767 |
|
BsmAI |
2 |
1265(c) 2828 |
|
BsmFI |
2 |
558 1574 |
|
Bsp120I |
1 |
351 |
|
Bsp1286I |
11 |
251 355 706 1369 1462 1536 1726 1788 2226 2311 3472 |
|
BspHI |
1 |
3062 |
|
BspMI |
3 |
1081(c) 1310(c) 1691 |
|
BspWI |
26 |
11 95 139 335 359 505 535 568 570 612 639 669 975 1114 1414 1498 1521 1660 1666 1783 1819 1866 2776 3164 3736 3850 |
|
BsrBI |
5 |
188 332(c) 633(c) 2036(c) 3853(c) |
|
BsrDI |
3 |
1656 2660 2834(c) |
|
BsrFI |
4 |
672 1742 1923 2809 |
|
BsrI |
20 |
82 273(c) 327 394 420 866 1005(c) 1052 1133 1217 1367 1568 2249 2419(c) 2688 2731 2849 3255 3367(c) 3380(c) |
|
BssHII |
1 |
1820 |
|
BstNI |
6 |
139 427 1810 3623 3636 3757 |
|
BstUI |
13 |
31 33 549 573 593 885 1489 1790 1822 2385 2828 3158 3739 |
|
BstXI |
2 |
280 322 |
|
BstYI |
11 |
253 1236 1259 1594 1840 2247 2264 3032 3044 3130 3141 |
|
CfoI |
31 |
5 33 98 526 551 564 573 595 621 629 969 1079 1177 1248 1417 1425 1489 1526 1792 1822 1824 2052 2385 2672 2765 3158 3267 3441 3541 3608 3878 |
|
Cfr10I |
4 |
672 1742 1923 2809 |
|
Csp6I |
3 |
242 1728 2412 |
|
DdeI |
4 |
2393 2933 3099 3508 |
|
DpnI |
26 |
255 505 1238 1255 1261 1266 1285 1596 1674 1755 1764 1842 1853 2213 2249 2266 2524 2570 2588 2929 3034 3046 3124 3132 3143 3218 |
|
DpnII |
26 |
253 503 1236 1253 1259 1264 1283 1594 1672 1753 1762 1840 1851 2211 2247 2264 2522 2568 2586 2927 3032 3044 3122 3130 3141 3216 |
|
DraI |
3 |
2316 3008 3027 |
|
DraII |
1 |
351 |
|
DraIII |
1 |
780 |
|
DrdI |
3 |
824 1450 3680 |
|
DsaI |
1 |
1855 |
|
DsaV |
13 |
137 425 1028 1425 1585 1808 2356 2378 2707 3403 3621 3634 3755 |
|
EaeI |
9 |
37 267 327 391 1329 1503 1894 1921 2501 |
|
EagI |
3 |
267 327 1329 |
|
Eam1105I |
1 |
2894 |
|
EarI |
5 |
484(c) 1767(c) 1977(c) 2095(c) 3899(c) |
|
Ecl136II |
1 |
249 |
|
EclXI |
3 |
267 327 1329 |
|
Eco57I |
4 |
1569 2001 2228 3240(c) |
|
EcoO109I |
1 |
351 |
|
EcoRI |
2 |
284 300 |
|
EcoRII |
6 |
137 425 1808 3621 3634 3755 |
|
EcoRV |
1 |
312 |
|
Fnu4HI |
38 |
54 270 327 330 454 527 560 574 596 976 1229 1332 1384 1395 1485 1490 1527 1568 1655 1658 1661 1897 1993 2034 2048 2150 2474 2501 2840 3168 3374 3377 3442 3585 3740 3858 3861 3879 |
|
FnuDII |
13 |
31 33 549 573 593 885 1489 1790 1822 2385 2828 3158 3739 |
|
FokI |
9 |
445(c) 1045 1228 1290 1747 1772 2455 2742 2923 |
|
FspI |
3 |
525 1525 2671 |
|
HaeII |
6 |
6 622 630 1178 1426 3542 |
|
HaeIII |
19 |
39 269 329 353 393 495 785 927 1331 1505 1896 1923 2503 2770 2850 3308 3742 3760 3771 |
|
HgaI |
4 |
555 2362 3092(c) 3670(c) |
|
HgiAI |
6 |
251 1536 1726 2226 2311 3472 |
|
HhaI |
31 |
5 33 98 526 551 564 573 595 621 629 969 1079 1177 1248 1417 1425 1489 1526 1792 1822 1824 2052 2385 2672 2765 3158 3267 3441 3541 3608 3878 |
|
HinP1I |
31 |
3 31 96 524 549 562 571 593 619 627 967 1077 1175 1246 1415 1423 1487 1524 1790 1820 1822 2050 2383 2670 2763 3156 3265 3439 3539 3606 3876 |
|
HindIII |
1 |
235 |
|
HinfI |
10 |
42 372 825 847 1908 2042 2895 3412 3808 3883 |
|
HpaII |
23 |
160 673 988 1030 1157 1328 1405 1427 1455 1586 1676 1743 1924 2357 2379 2599 2709 2776 2810 3214 3404 3430 3577 |
|
HphI |
6 |
777 2167(c) 2202 2408(c) 2824 3051 |
|
ItaI |
38 |
54 270 327 330 454 527 560 574 596 976 1229 1332 1384 1395 1485 1490 1527 1568 1655 1658 1661 1897 1993 2034 2048 2150 2474 2501 2840 3168 3374 3377 3442 3585 3740 3858 3861 3879 |
|
KasI |
2 |
1174 1422 |
|
KpnI |
1 |
245 |
|
Ksp632I |
5 |
484(c) 1767(c) 1977(c) 2095(c) 3899(c) |
|
MaeI |
7 |
260 346 624 1124 2701 3036 3289 |
|
MaeII |
11 |
407 666 776 819 831 995 1539 1726 2292 2665 3081 |
|
MaeIII |
14 |
262 411 431 587 599 1543 2233 2421 2574 2632 2963 3246 3362 3425 |
|
MamI |
3 |
314 1258 1282 |
|
MboI |
26 |
253 503 1236 1253 1259 1264 1283 1594 1672 1753 1762 1840 1851 2211 2247 2264 2522 2568 2586 2927 3032 3044 3122 3130 3141 3216 |
|
MboII |
11 |
10 501 638(c) 1784 1994 2074(c) 2112 2221 2299 3054 3125(c) |
|
McrI |
9 |
270 330 506 746 1332 2376 2525 3448 3872 |
|
MluNI |
1 |
1505 |
|
MnlI |
18 |
32 342(c) 485(c) 750 1189 1274(c) 1338(c) 1474(c) 1831(c) 2024 2521(c) 2727(c) 2874 2955 3355 3605(c) 3679 3888(c) |
|
MscI |
1 |
1505 |
|
MseI |
16 |
48 107 293 442 567 838 936 953 2315 2680 2719 2954 3007 3021 3026 3078 |
|
MslI |
4 |
1860 2123 2482 2641 |
|
MspA1I |
7 |
56 475 1169 1529 2258 3199 3444 |
|
MspI |
23 |
160 673 988 1030 1157 1328 1405 1427 1455 1586 1676 1743 1924 2357 2379 2599 2709 2776 2810 3214 3404 3430 3577 |
|
MvaI |
6 |
139 427 1810 3623 3636 3757 |
|
MvnI |
13 |
31 33 549 573 593 885 1489 1790 1822 2385 2828 3158 3739 |
|
MwoI |
26 |
11 95 139 335 359 505 535 568 570 612 639 669 975 1114 1414 1498 1521 1660 1666 1783 1819 1866 2776 3164 3736 3850 |
|
NaeI |
2 |
674 1925 |
|
NarI |
2 |
1175 1423 |
|
NciI |
7 |
1030 1427 1587 2358 2380 2709 3405 |
|
NcoI |
1 |
1855 |
|
NdeII |
26 |
253 503 1236 1253 1259 1264 1283 1594 1672 1753 1762 1840 1851 2211 2247 2264 2522 2568 2586 2927 3032 3044 3122 3130 3141 3216 |
|
NgoMI |
2 |
672 1923 |
|
NlaIII |
14 |
226 342 1116 1297 1642 1828 1859 1885 2451 2487 2565 2575 3066 3786 |
|
NlaIV |
17 |
134 243 255 353 705 717 738 986 1176 1424 1459 2597 2808 2849 2943 3715 3754 |
|
NotI |
1 |
327 |
|
NsiI |
1 |
344 |
|
NspI |
3 |
342 1828 3786 |
|
PaeR7I |
1 |
333 |
|
PleI |
6 |
380 833 841(c) 2903 3406(c) 3891 |
|
Ppu10I |
1 |
340 |
|
Psp1406I |
2 |
2292 2665 |
|
PstI |
2 |
309 1476 |
|
PvuI |
2 |
506 2525 |
|
PvuII |
4 |
56 475 1169 1529 |
|
RcaI |
1 |
3062 |
|
RsaI |
3 |
243 1729 2413 |
|
RsrII |
1 |
1939 |
|
SacI |
1 |
251 |
|
SapI |
3 |
1767(c) 1977(c) 3899(c) |
|
Sau3AI |
26 |
253 503 1236 1253 1259 1264 1283 1594 1672 1753 1762 1840 1851 2211 2247 2264 2522 2568 2586 2927 3032 3044 3122 3130 3141 3216 |
|
Sau96I |
9 |
351 352 494 783 1939 2530 2752 2769 2848 |
|
ScaI |
1 |
2413 |
|
ScrFI |
13 |
139 427 1030 1427 1587 1810 2358 2380 2709 3405 3623 3636 3757 |
|
SfaNI |
11 |
351 1381(c) 1636(c) 1722 1786 1852(c) 2061 2193(c) 2442 2633(c) 3685(c) |
|
SfcI |
7 |
305 365 554 1472 2648 3326 3517 |
|
SnoI |
2 |
2222 3468 |
|
SpeI |
1 |
259 |
|
SphI |
2 |
342 1828 |
|
StyI |
2 |
1232 1855 |
|
TaqI |
8 |
334 742 1536 1692 1716 1752 2240 3684 |
|
TfiI |
4 |
42 1908 2042 3808 |
|
ThaI |
13 |
31 33 549 573 593 885 1489 1790 1822 2385 2828 3158 3739 |
|
Tru9I |
16 |
48 107 293 442 567 838 936 953 2315 2680 2719 2954 3007 3021 3026 3078 |
|
Tsp509I |
13 |
104 179 196 284 300 356 383 958 1160 2088 2461 2716 3022 |
|
Tth111I |
1 |
1541 |
|
XbaI |
1 |
345 |
|
XcmI |
1 |
1239 |
|
XhoI |
1 |
333 |
|
XhoII |
11 |
253 1236 1259 1594 1840 2247 2264 3032 3044 3130 3141 |
|
XmaIII |
3 |
267 327 1329 |
|
XmnI |
1 |
2294 |
No cuts: AatI, AatII, AccI, AccIII, AgeI, AscI, AsuII, AvrII, BbrPI, BbsI, BlnI, Bpu1102I, BpuAI, BseAI, BsgI, BsiWI, BsmI, BspDI, BspEI, BsrGI, Bst1107I, BstBI, BstEII, Bsu36I, CelII, ClaI, Csp45I, Eco47III, EcoNI, Esp3I, EspI, HincII, HindII, HpaI, KspI, MfeI, MluI, MroI, MunI, NdeI, NheI, NruI, NspV, PacI, PflMI, PinAI, PmaCI, PmeI, PmlI, PpuMI, SacII, SalI, SexAI, SfiI, SfuI, SgrAI, SmaI, SnaBI, SspBI, SspI, StuI, SwaI, Van91I, XmaI
Sequence from GV-1 Oligo:
CGTTGTAAAACGACGGCCAGTGCCAAGNCTTGCATGCCTGCAGGTCGACTCTAGGAGGATCCCCGGGTACCGAGCTCGAA
TTCGCGCCCGGGGTATCCTCTAGCGCCATGGCACCCCTGAGACCCCTTCTCATACTGGCCCTGCTGGCATGGGTTGCTCT
GGCTGACCAAGAGTCATGCAAGGGCCGCTGCACTGAGGGCTTCAACGTGGACAAGAAGTGCCAGTGTGACGAGCTCTGCT
CTTACTACCAGAGCTGCTGCACAGACTATACGGCTGAGTGCAAGCCCCAAGTGACTCGCGGGGATGTGTTCACTATGCCG
GAGGATGAGTACACGGTCTATGACGATGGCGAGGAGAAAAACAATGCCACTGTCCATGAACAGGTGGGGGGCCCCTCCCT
GACCTCTGACCTCCAGGCCCAGTCCAAAGGGAATCCTGAGCAGACACCTGTTCTGAAACCTGAGGAAGAGGCCCCTGCNC
CTGANGTGGGCGCCTCTAANCCTGAGGGGATANACTCAAGGCCTGANACCCTTCATCCAAGGAGACCTCANNCCCCAGCA
NAAGANGAGCTGTGCAGNGGGAANCCCTTNGACGCCTTCACCGACCTNAAGAACGGTTCCCTCTTTNCCTTNCNAGGGCA
GNNCTGCTATNAACTGGAACNAAAANGCANNGAGGCCTGGGTACCNCAANCTCATNCCANANGNNTGGGGCNTCNANAGG
CCCCATNNANNCCCCCTTNACCCNCNNNAACTNTNAGNGGNAAGANCTANNTNTTCANGGTTNNNNNNNCTGNCCCNTTN
NANANNNNGNNCCNGNCCCNGATNCCCCNAANATNNNTNACNGNTNNANGNNNNCCCNNNAACGGNANCNGNNTNNGCNT
CCTNCCNNNNNANNNNNNCNNNANNGGNTNNTTTTTNAGNGGAANANNNNNGNNNCCCNNCCGNNCNNNC
Sequence from GV-2 Oligo
ACAGCTATGACATGATTACGAATTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGGGCTAANGGACCNTTA
TTGGGCTGGGGGAANGAAANGGGAGGANGGACCNCCCAAGGGCCCCGCCATNTGGGCNCNNACTCCTACAAATGGCCANN
ACCTGGCCNCCCCANCCAATNCNNACCNATNNANCCTGGGTANGNANNNCCCNCAGNGNCCNCNCCCCNAGNGCCAANAN
GGACNCNANNGNCCTTNACNCCCCAAAAAAAAAAAACCCNCCNGCTNGNNTCACAGCTCACAACCCCANCCCNGCCCANC
CTGTGANCANNANAANTGCTCCCNCCCAANAAGCTCNCCCNNCNTNAAAACAANCACNGCCNCNTANANCCCNAAAACNC
GNCAAAANGTCNNGTTACGCNCNCANAAANAACNCCNGCNTAAACNGAAAACCGNCCACCACAANATNTCAAAACCTTNN
GTCCCTAACCCAAGCGCGCACCAGACCNCNAATCAAAAAAANAAANGCCCCGGCNANNCNGCCCAAACCNCTAACCCTNC
CNACCANTGCCNAACCNNCCAANCANAANGNNCCTNACAANACCCCNAANCNNCCACCCCCCNAAAACNNNCCNCCGAAA
CCCCCGCCCACGGCNCNCCAGCCTCCTNAAAAATANCACNNNCANNCCNNACNNCCNNGANACCGNNNCCCCNANNNNNA
AANACGCCCAAAANCANCCNCCCCCNAGNCACNCCNNNGCAANANCCACCNNCGCCNGCGANCAACNAAAANANANGNGG
CGNNGGCCCGGAGACCNGNACCCNACANCCCNAAANCNCANGCGNNCCCNNNANGNCCNNNANACACCCCNCGNCCCAAA
GNNNAGCNACNCNCNCCNNNNGNCANANANAGNNGCANANCAANCNCCCT
Sequence from GV-1 Oligo:
TAAAACGACGGCCGTGCCAAGCTTGGCTGCAGGTCGACGGATCCGTCCCGGATAAGACGCGACCGGCGTCGCATCCGGCG
CTAGCCGTAAATTCTATACAAAATTACCGCCGCTCCAGAATCTCAAAGCAATAGCTGTGAGAGTTCTGCGCATCAGCATC
GTGGAATTCTATTAGTTGATGCGTTCAAGCGCCGCAACAGGATAGATCTGTACTGAGCCCGGGTGAGCCTCAGACTCGAC
GGCGTAGCCCTCAGGGGTCAAATTTGTGCAGTACCACCCGACAATCTGACCTTGCCAGGCGGCGCCGGATTTCTTACGTA
CGCGGTCACCCATACCAAACGTGGCGTTCGATGGGAATACAAAATTGCCGGCAACTGGATTCGAAACTTCATTAGAGCTC
CTGATCATTGAGATTTCCCGATAAAAAAAATTGTCGCCACTATACGTAAAGCGTAAACCGTCGTCAACTGGTGCGAGGAT
GATGTTGAGGAAAATTTTATATTCTGCTGGCGAGTCCACGCTCTCTCCCTGGACTCGCCGCATTACAATGAAACAAAAAC
AAACAGTTAGCTGTAAAGTGTGATTTACGTCACTCTTTATTAGGATGAGGGTTTCGTTTCCGGTTCATCCGCCATGTTGN
CGGTATGTTTACCTTCTTCGGNTCCCTGCCAGCCATGTCGTTGAATNAATGACAGATGTTCGCGGGCCTCGGTAATGATC
TCACTTAACATCGCGCTGGTGCGTTTATANACCNCCGCGCGGGCTTTGGNGNCGNTNTCAANCATTGCCATTTCTTNCCC
CATTTGAATNTNAAAGGGGNGGAACACATTGGGNCGTTNTCGCGCTTTANCCGGNCCCAAACCTAAACTTTTCAAGGCCA
AACCCCNGGTTTTAACGCNCNTNCNAGGGTTNACCCNCC
Sequence from GV-2 Oligo
CAGCTATGACCTGATTACGAATTCCCGGGGATCCGTCGACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGG
AGCGTGAAACCTTCGAAGGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGTCTGGGGCCGTATGAAGCGCGA
GAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATGGTTGAGAACGACACCAAAGCCCG
CGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGTGAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTCAAC
GACATGGCTGGCAGGGAACCGAAGAAGGTAAACATACCGGCAACATGGCGGATGAACCGGAAACGAAACCCTCATCCTAA
TAAAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGC
GTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAG
TGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGGAGCTCTNATGAAGTTTCGAATCCAGTTGCCGGCAATTTTT
GTATTCCCATCGAACGCCACGTTTGGNTATGGGTGACCGCGTACGTAAGAAATNCCGGCGCCCCCTGGCAAGGTCANATT
GTCNGGGGGTACTGCACAAATTTGACCCCTGAGGGCTACNCCCGNCGAGTNTGAGGNTCCCCCGGGNTNAANACAGATTT
ATTCTGTTGGNGGGNNTTGANCCCTNCAACTAATAGAATTNCCCGATGNTGGTGCGCANAACTCTCACAGNTNTTGNTTT
GNAANTNTNGGGCNGNCGGAATTTTGTNNAAAANTACCGGTAACGCCNGNTGCCNCC
Sequence from GV-1 Oligo:
GTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGCCCTCTAGATGCATGCTCGAGCGGC
CGCCAGTGTGATGGATATCTGCAGAATTCGGCTTGTCGACCCCATGGCAGAGGAGGAGCTGTGCAGTGGGAAGCCCTTCG
ACGCCTTCACCGACCTCAAGAACGGTTCCCTCTTTGCCTTCCGAGGGCAGTACTGCTATGAACTGGACGAAAAGGCAGTG
AGGCCTGGGTACCCCAAGCTCATCCGAGATGTCTGGGGCATCGAGGGCCCCATCGACGCCGCCTTCACCCGCATCAACTG
TCAGGGGAAGACCTACCTCTTCAAGGGTAGTCAGTACTGGCGCTTTGAGGATGGTGTCCTGGACCCTGATTACCCCCGAA
ATATCTCTGACGGCTTCGATGGCATCCCGGACAACGTGGATGCAGCCTTGGCCCTCCCTGCCCATAGCTACAGTGGCCGG
GAGCGGGTCTACTTCTTCAAGGGGAAACAGTACTGGGAGTACCAGTTCCAGCACCAGCCCANTCAGGAGGAGTGTGAAGG
CAGCTCCCTGTCGGCTGTGTTTGAACACTTTGCCATGATGCAGCGGGACAGCTGGGANGACATCTTCGAGCTTCTCTTCT
GGGGCAGAACCTCTGCTGGTACCAGACAGCCCCAGTTCATTANCCCGGGNACTGGCACNGTGTGCCAGGGCAAGTGGACT
GCGCNATGGCTTTAAAANCCGAATTCCACCACACTGGCGGNCGTTACTATTGGATCCNAGCTCGGNCCCANCTTGGCGAA
ACATGGGCATANCTGNTTCCTGTGNGAAAATGTNTTCCNTTCNAANTTCCCNNAAANNNNANCCGGAAACTAAAANGTAA
ANCCTGGGGNGCCTNNAGAGNGANCTNACTCCNTT
Sequence from GV-2 Oligo
AGCTATGACCTGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTT
TTAAGCCATTGCGCAGTCCACTTGCCCTGGCACACCGTGCCAGTCCCGGCTAATGAACTGGGGCTGTCTGGTACCAGCAG
AGGTTCTGCCCCAGAAGAGAAGCTCGAAGATGTCCTCCCAGCTGTCCCGCTGCATCATGGCAAAGTGTTCAAACACAGCC
GACAGGGAGCTGCCTTCACACTCCTCCTGACTGGGCTGGTGCTGGAACTGGTACTCCCAGTACTGTTTCCCCTTGAANAA
GTAGACCCGCTCCCGGCCACTGTAGCTATGGGCAGGGAGGGCCAAGGCTGCATCCACGTTGTCCGGGATGCCATCGAAGC
CGTCAGAGATATTTCGGGGGTAATCAGGGTCCAGGACACCATCCTCAAAGCGCCAGTACTGACTACCCTTGAAGAGGTAG
GTCTTCCCCTGACAGTTGATGCGGGTGAAGGCGGCGTCGATGGGGCCCTCGATGCCCCAGACATCTCGGATGAGCTTGGG
GTACCCAGGCCTCACTGCCTTTTCGTCCAGTTCATAGCAGTACTGCCCTCGGAANGCAAAGAGGGAACCGTTCTTGANGT
CGGTGAANGCGTCAAAGGGCTTCCCACTGCACAGCTTCTTCTNTGCCATGGGGTCGACAAGCCGAATTCTGCAGATATNC
ATCACACTGGCGGNCGNTCGAGCATGCATTTAGAGGGNCCNATTCNCCCTATAGNGAGTCGTNTTACAATTCACTGGCCG
GCGTTTTACAACGTNNGNGACTGGGAAAACCCTGGNGTTNCCNANNTNATTGCCTTGGAAAAATNCCCCTTTTNCCAGCT
GGGGTATAACNAAAANGNCCCCNCNATCNCCNTTTCCANANTTGCCCACCTTANGGGAAANNNCCCCCCNTTANCGG
Sequence from GV-1 Oligo:
sequencing failed...
Sequence from GV-2 Oligo
CAGATTCTGGCTGATCAGCTGACTGACGAGCAGATCGCTGAATTTAAAGAGGCTTTCTCTCTGTTTGACAAAGACGGTGA
CGGTACCATCACTACCAAAGAGCTCGGCACCGTTATGCGCAGCCTTGGCCAGAACCCGACTGAAGCTGAACTGCAGGACA
TGATTAACGAAGTCGACGCTGACGGTAACGGCACCATCGATTTTCCGGAATTTCTGAACCTGATGGCGCGCAAGATGAAA
GACACTGACTCTGAAGAGGAACTGAAAGAGGCCTTCCGTGTTTTCGACAAAGACGGTAACGGTTTCATCTCGGCCGCTGA
ACTGCGTCACGTTATGACTAACCTGGGTGAAAAGCTTACTGACGAANAAGTTGACGAAATGATTCGCGAAGCTGACGTCG
ATGGTGACGGCCAGGTTAACTACNAAGAGTTCGTTCAGGTTATGATGGCTAAGTAGGATCCTGGCGTAATAGCGAAGAGG
CCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAT
CTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACA
CCCGNCAACACCCGNTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTNCG
GGAGCTGCATGTGTCAAANGGTTTCACCCGTCATCACCGAAACGCGCGAAACAAAAGGGCCTCGGGANNCNCCTNTTTTT
TAGGGTAATGGCATGATAAAANGGGNTTTTAAACCCCAGGGGGNNNTTTTNGGGNAANGTGCNNGGANCCCNTTTTGNTT
TTTTTNTAAAACNTNNAAANTGTTNCNCTCAGNAAAAANACCCTGNNAATGCTTNATAN
Sequence from GV-1 Oligo:
TTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGCCCTCTAGATGCATGCTCGAGCGGCC
GCCAGTGTGATGGATATCTGCAGAATTCGGCTTGTCGACCCCATGGCAGAGGAGGAGCTGTGCAGTGGGAAGCCCTTCGA
CGCCTTCACCGACCTCAAGAACGGTTCCCTCTTTGCCTTCCGAGGGCAGTACTGCTATGAACTGGACGAAAAGGCAGTGA
GGCCTGGGTACCCCAAGCTCATCCGAGATGTCTGGGGCATCGAGGGCCCCATCGATGCCGCCTTCACCCGCATCAACTGT
CAGGGGAAGACCTACCTCTTCAAGGGTAGTCAGTACTGGCGCTTTGAGGATGGTGTCCTGGACCCTGATTACCCCCGAAA
TATCTCTGACGGCTTCGATGGCATCCCGGACAACGTGGATGCAGCCTTGGCCCTCCCTGCCCATAGCTACAGTGGCCGGG
AGCGGGTCTACTTCTTCAAGGGGAAACAGTACTGGGAGTACCAGTTCCAGCACCAGCCCANTCAGGAGGAGTGTGAAGGC
AGCTCCCTGTCGGCTGTGTTTGAACACTTTGCCATGATGCANCGGGACAGCTGGGANGACATCTTCGAGCTTCTCTTCTG
GGGCAGAACCTCTGCTGGTACCAGACAGCCCCAGTTCATTANCCGGGACTGGCACNGTGTGCCAGGGCAAGTGGACNCAA
CCATGGGTNGGCCNCATNTACATNTNANGGGTNGGNCCCCCCCCCNTNCTTGGNCNAAAAAAAAAAGGNTTTAGGGATTN
NAANCCNCAAAGGGTNNCCNTTNNAANAAAGGNCNCANNCGGGGGGCGNAAACAAAAATTCCGNCNGGNCTTCCCCNNCC
ANGNGGGTGNTCNTTTTTTTCCAAGGNNGNNNAANANNTTNGGGANCCCAN
Sequence from GV-2 Oligo
AGCTATGACCTGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTT
GGCTCTGACTCCTACAGATGGCCAGGAGCTGGGCAGCCCAGCCAGTACTGAGCGATGGAGCGTGGGTAGGGAGGGTCCAC
AGTGTCCACTCGCCGTGTGCGAAGATTGACTCGGTAGTACTTGTCTCCAGAGAAGAAGAAGACACTCTGGATGGGTTCAC
AGGTGGCAGGCACAAGCCAGTCCATCCTGTAGTCATCATAGTTGTTGGCTCCCAAGTTGCTCTCCTCACTGGAGAACAAG
GACAGCCACGTGGCGCGGGATGGCCGGCGGGAGTTCTGGTTGCGGCCACGGCTGTGGCCTCGTTGTGAACGGTAGCCTTT
GCGGTTGCGATGCCTAAACCTTTGTTTCTTGGCCAAGGAGGGGCGGGGTGCCATGCCTGAGATGTAGATGCGGCCAGCCA
TGGCTGCGTCCACTTGCCCTGGCACACCGTGCCAGTCCCGGCTAATGAACTGGGGCTGTCTGGTACCAGCAGAGGTTCTG
CCCCAGAAGAGAAGCTCGAAGATGTCCTCCCAGCTGTCCCGCTGCATCATGGCAAAGTGTTCAAACACAGCCGACAGGGA
GCTGCCTTCACACTCCTNCTGACTGGGCTGGTGCTGGAACTGGTACTCCCANTACTGTTTCCCCTTNAAAAATANACCCN
CTCCCCGCCACTGTANCTNTGGGCAGGGAGGGCCANGNTNCNTCCCCTTGTCCGGGATGCCTTNNAACCCCCANANATTT
TTCGGGGGNATNAGGGNCCAAAAANCATTCTTAAANGCCNGTACTGACTNCCTTTNAAANGGNGGTNTNCCCTCANNNTT
NTGCNGGNGAAGCGGGNTTTNTGGGGCCCTTATNCCCNNAATTTTGGAAANNTTGGGGNCCCNC
Sequence from GV-1 Oligo:
GTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGCAGGATCCCCGGGTACCCGGCTCGAATT
CTCATGTTTGACAGCTTATCATCGATAAGCTTGGGGTAACGCAATCAGTATTGTTCATTTTTTTTCCCGGAAAAGCTAAG
AGTTTTCTGGCGAGATAAGATAACGGCGCCGAAGGCAGCGGCGAACTGACTTACCTCCGTCGGCTCCGGCGTACGGCACC
AAAAAATCCCGCAATAACTTGACACCAATGGGCTAACGGCGTCCGCAGAACCGTGACGCAATCTGCGGTTTGTGTCAAGA
CAGAGAAGTGGGCTGCTTCTCCCCTTTCAACACTCCCCTCCCATGGCTGAATACTCAGACGTTTTCTGTGGTGGAAATGG
CATCAAAAGTGCCACTGGCTGGCGAATCCAGACTATTTTGTGTCAAANAAACATGCGTACCTGCACAATAAAAAGAAAAG
GCCCGCCCTACTGACATCCTGTTCAGGATCCTCGAAGAACAGGAAAAAGGACAACCGTCCTTTTCACTTCACGTTCGCAG
GGCTTTCCTCGATATAATGGCGATTACACAAGCGGTGGCGTCTTGAAAAAAGTTGGGTTTCTTATCTGTACNGCGATCAC
TCCCCTCATATGATAGCTGTGTACAGTGACTTGCTCTTTGATTGGCCTTACTTCTTGCATCTATCATNCCTTTCTTTTAC
TCTNTCCATCACCACAACTNATATCATTCTACNTTTCATNTTATTGGTCTGNNCAATNTTTAGGGTGNAAAAAATATAAG
GGNNGTCNTATTTTCTNNTNCTTTCCGNTGGGGCNNTTTTAGGGGTGGCTTATTACCATTTTCCTACTTATTTTTACTGC
NTTANTTNAAAAAAGGNTNCCCNNNGCTGTTCCCTGAAAAAT
Sequence from GV-2 Oligo
CAGCTATGACATGATTACGAATTCCATGTAATTCCATCATACTGGCCTGNGTGATGAAACTGGAAATCATGGTACCGTAT
TGGGTCCAATAAATTGGGTAGAATATAAAGACTTTACAAGCCGCCAAAGCTCTCTTAATTTCATCAACAAATTTGTCATT
CCATGGATAGTTCTTTTCTGGATGAACAGAAGGTTTAGCAGCGTTGAAGTCGAACTTATTCTTAGTTAAAATCCAACAAA
CTTTAAAACTTTTAGCGATGACTTTATCTCCGATTGGTCTTTGAATGTATTGCTTTTTACCAAAAATCAAAGTGACAACA
GCGATCCAAAAGAAGCAGAAGGGCAATAGATAGGCCGCCCAGAACCCCTTATGATATTCCAATTCAGTAGTGGCCATTAA
TGATAGAGAACCGACATTAATCATGAAATAGGCGAACATAAAAACGTTTTGTAAAGTAATATTTGAATCGACAATGACTC
TTTCGCCCGATTTTAAAACTTTGATGGAGGGTTTCCGCTTAGGAAGCTGGTCGGCAATCAACACGGAAAGGTTANCTTTA
ATCATACCAGTGGCAATACCGATCAGAATAATGGCAGCAATGAACCCACCAATAGCACTGTCTCTGTTACCAACGGAGGG
NATCGAAGTGATAAATAGAATGAAANTNCCGGCAATGTAAATAGGCGGTNCCGCCANCAAATTGTNTTATATTTACCCNA
NANGGTGTCCCCCACGNNACCNCCNAAAACTGGTNGTNACCNTACACCAAAACTGGAAAAAATACGACACCCCANTGGGC
CCCCTGNACNGNTNAAGNNCAAAACACCCTTTGGGGAGNAATNAGGNCCNTTTNCATNTANNTTTGGNAAGGGGGCGAAA
ANCCCCNANTAGGANAANTNNNGGGAAGCTTNCCATANCAATNACCNNCCCTCNTAGGAANTTTNCNCCC